ʻO ka maʻi Bovine Rhinotracheitis Virus Antibody ELISA Detection Kit

Helu Kumu:VD105E
Abbr.:IBRV Ab Elisa
Hoʻohālikelike: serum

Hōʻike

Hōʻike

BVDV Ab Elisa

Hoohana Manao: HoʻopiliʻO ka maʻi maʻi Rhinotracheitis BovineHoʻohana ʻia ʻo Antibody ELISA Detection Kit ee hoʻoholo i ka maʻi rhinotracheitisvirus antibodies i loko o ka bovine serum in vitro.

Hoʻohālike: serum

Nā mea i hāʻawi ʻia:

Infectious Bovine Rhinotracheitis Virus Antibody ELISA Detection Kit

Nā Kaʻina Hana a me ka wehewehe ʻana

KANAWAI 1 Ke kaʻina hana hoʻāʻo

1.1 Holoi Hoʻomākaukau

Working concentration wwai lehu:Ma mua o ka hoʻohana ʻana, pono e hoʻihoʻi ʻia ka wai holoi i ka lumi wela (20-25 degere) a hoʻoluliluli like ʻia e hana like. A laila e hoʻoheheʻe i 20 mau manawa i ka wai holoi holoi me ka wai i hoʻomaʻemaʻe ʻia (no ka laʻana, hoʻohui ʻia ka 5ml wai holoi hoʻomaʻemaʻe i 95ml wai i hoʻomaʻemaʻe ʻia), hui maikaʻi, a hiki ke mālama ʻia ka wai holoi holoi ʻia ma 2-8 degere no 7 mau lā.

1.2 Hoʻomākaukau laʻana

① Lawe ʻia ke koko holoholona holoʻokoʻa, centrifuged ma 4000rpm no 10 mau minuke, a ʻohi ʻia ka supernatant. Hiki ke hoʻopili maoli ʻia ka serum me ka hemolysis ʻole.

② E hoʻoheheʻe i ka serum e hoʻāʻo ʻia i nā manawa 50 i ka pā dilution serum (no ka laʻana: hoʻohui i 3ul o ka serum e hoʻāʻo ʻia i 147ul sample diluent).

Nānā: ʻAʻole pono e hoʻoheheʻe ʻia nā mana maikaʻi ʻole a maikaʻi ʻole

1.3 Kaʻina hana hoʻāʻo

①E lawe i ka lepili enzyme Microplate (e like me ka helu o nā laʻana, hiki ke hoʻokaʻawale ʻia a hoʻohana ʻia i nā manawa he nui), e hoʻohui i 100ul o ka serum i hoʻoheheʻe ʻia i ka inoa enzyme Microplate, e hoʻonoho i ʻelua pūnāwai no ka mana maikaʻi ʻole a me ka mana maikaʻi, a hoʻohui i 100ul i ka hopena maikaʻi. E lulu mālie i ka hāpana i loko o ka pūnāwai, me ka hoʻohana ʻana i ke kiʻi ʻoniʻoni paʻa e uhi i ka pā, a hoʻomoʻa ma 37 degere no 30 mau minuke.

②Wehe i ke kiʻi ʻoniʻoni pāpaʻi sila, lulu i ka hopena i loko o ka pā maikaʻi, hoʻohui i 300µl o ka wai holoi holoi hana i kēlā me kēia luawai, a laila e hoʻolei i ka wai i loko o ka luawai. E hana hou i nā ʻanuʻu ma luna aʻe 5 mau manawa, a ma hope e hoʻomaloʻo i kahi pepa maʻemaʻe.

③Diluted enzyme conjugate: E hoʻoheheʻe i ka enzyme conjugate (80×) 80 manawa (no ka laʻana: 10ul enzyme conjugate concentrate + 790ul sample diluent, ua helu ʻia ka nui kikoʻī e like me ka helu o nā laʻana hoʻāʻo), hui maikaʻi a waiho ʻia.

④E hoʻohui i 100µl o Enzyme Conjugate i kēlā me kēia pūnāwai, e uhi i ka pā, a e hoʻomoʻa i ka 37 degere no 30 mau minuke.

⑤Ka holoi ʻana i ka pā enzyme, ʻo ka hana kikoʻī e like me ka mea i luna ②.

⑥Hoʻohui i 100µl substrate solution i kēlā me kēia pūnāwai, e uhi i ka pā a hoʻomohu no 10 mau minuke ma 37 degere ka mamao mai ka mālamalama.

KANAWAI 2 Huna Huli

Ua heluhelu ʻia ka waiwai OD450 o kēlā me kēia pūnāwai ma ka mea heluhelu microplate 96-well.

2.1 Hōʻoia ʻO ka awelika OD450nm waiwai o ka mana maikaʻi ʻole he <0.25 a ʻo ka awelika OD450nm waiwai o ka mana maikaʻi ʻoi aku ka nui ma mua a i ʻole like me 0.6, ua kūpono nā hopena hoʻokolohua. A i ʻole, pono e hana hou i ka hoʻāʻo.

2.2 Heluhelu

No kēlā me kēia laʻana, e helu i ka S/P e like me kēia me ka hoʻohana ʻana i ka laʻana i hoʻoponopono ʻia a me nā waiwai mana:

product-554-49

2.3 Ka Huli Huli

Inā ʻo S/P <0.2, manaʻo ʻia he ʻino.

Inā ʻo S/P > 0.3, manaʻo ʻia he maikaʻi.

Ke emi mai ka 0.2 a i ʻole like me S/P I lalo a i ʻole like me ka 0.3, ua manaʻo ʻia he kānalua, a manaʻo e hana hou i ka laʻana a hoʻāʻo i hoʻokahi pule ma hope.

Nā huahana pale: kit ʻike maka ʻana o ka bovine rhinotracheitis virus antibody elisa detection kit, Kina kit infectious bovine rhinotracheitis virus antibody elisa detection kit mea hoʻolako, mea hana, hale hana

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